Standard conditions for food, additives, etc. (excerpt)
Condition 1: Food
Each condition
○ Food sterilization by heat pressure after packaging

Conditions on the composition of foods using retort technology (products that kill bacteria by heat treatment after packaging (other than purified water, meat products, whale meat, and products made from fish meat) ) are products that are packed into sterile packaging, then closed, and sterilized by heat).

Food using retort technology must meet negative internal microbiological conditions. Microbiological test method as below: 
(1 ) Test under stable temperature condition.

Take a sample of the item in its original packaging, then store it at a temperature of 35 degrees (accept a difference of about 1 degree) within 14 days. During this time, observe whether the packaging expands or leaks contents. Testing for expansion of the packaging is carried out after cooling the product to a temperature of 20 degrees Celsius. Samples of items that are confirmed to have no expansion or leakage of the packaging will be considered products. achieve negative internal microbiological conditions.  

Items that achieve a negative result after testing at a stable temperature will be tested for bacteria.

(2 ) Bacterial test

1.Adjust the test pattern

  For items with negative microbiological results after testing at a constant temperature, wipe the surface of the product packaging with an alcohol swab, and then open it with a sterilized instrument. After thoroughly mixing all the contents under sterile conditions (in case all or part of the contents are solid, cut them with sterile scissors), take 25g of the contents under sterile conditions. Add 225ml sterilized phosphate buffered saline (sterile buffered saline) and separate. Continue using a pipette (a laboratory glass straw for moving liquids) take 1ml of the item into a sterile test tube, then add 9ml of sterilized phosphate buffered saline, mix well, and remove the item to check.

2. Test method

  Take a sample of the test item, put it in 5 test tubes of thioglycolic acid, 1ml each, and then culture it for 48 hours (accept a difference of about 3 hours) at 35 degrees Celsius (the difference can be about 1 degree Celsius). If one of the samples is confirmed to have an increase in bacteria, the product is said to be positive.

  Add 0.5g L-cystine thioglycolate culture, 5g glucose, 5g yeast extract,15g  peptone, 0.5g thioglycolate, 2.5g salt, 0.001g resazurin and 0.8g powdered agar to 1,000 ml of purified water. Dissolve by heating, adjust pH 7.0-7.2, add 10 ml to each test tube and sterilize at 121° for 15 min.

Food production standards for sterilization using pressure cooking method. 1. The raw materials used for production (e.g., vegetables) must be fresh and of good quality. 2. The raw materials used for production (e.g., vegetables) must be thoroughly washed and meet the necessary standards. 3. No preservatives or chemical additives used for bacterial disinfection are allowed during the production process (except for hypochlorite and sodium hypochlorite). 4. Products packaged using the retort method (except for canned or bottled food) must have their packaging mouth sealed by heat or tightly rolled. 5. The heat sterilization process during production must be performed using a sterilization machine equipped with a self-recording thermometer, and the records must be kept for 3 years. 6. Heat disinfection during production must be carried out using a high-pressure heat sterilization method that satisfies the following conditions: - Effective enough to kill microorganisms originating from raw materials and capable of surviving and growing in food. - For products using the retort method with a pH above 4.6 or water activity (aw) exceeding 0.94, a method using a central temperature above 120 degrees for 4 minutes or an equivalent effective method must be used. 7. Water used for cooling after heat sterilization must be discharged underwater (using only production water) or continuously replaced with water containing free residual chlorine above 1.0 ppm. 8. Machinery used for production must be thoroughly cleaned and sterilized.

Article 3: Machinery and packaging
E – Standard conditions for production tools and packaging

Packaging for heat-sterilized food (except canned/bottled food) must meet all of the required conditions set out below. (For packages packed by the wrapping method, condition (4) does not apply)

(1) Must be light-blocking and impermeable. However, this condition does not apply if the product does not present a risk of deterioration due to fat modification.
(2) The product must not be damaged, deformed, discolored, discolored, etc., after being filled with water, sealed, and pressure-heated during production.
(3) When carrying out the stress test in the endurance test method, there shall be no leakage of internal substances or water.
(4) When carrying out the strength test of the mouth of the package sealed by heat sealing, the measured value should be 23N or more.
(5) When performing the drop test in the endurance test method, there should be no leakage of the item or the water inside. However, if the packaging is packaged for retail sale, the inspection must be carried out in the same condition as in the packaging used for retail purposes.